Electronic Library of Scientific Literature
Electronic Library of Scientific Literature
Volume 42 / April 1998 / number 2
T. YANASE, Y. HASHIMOTO, T. MATSUMOTO
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
Summary. – Viral DNA was extracted from cells infected with bombyx mori nucleopolyhedrovirus (BmNPV) D1 strain after 34 serial undiluted passages (P34). P34 DNA was subjected to restriction analysis and Southern blot hybridisation using standard D1 DNA and P34 DNA of BmNPV as probes. Based on hybridisation profiles, the BmNPV DNA regions retained in the P34 DNA were localised on HindIII and PstI restriction maps. Two regions of BmNPV DNA located at 0 – 12.8 and 40.2 – 65.0 map unit (m.u.) were highly conserved in P34 DNA. These regions contained two of three interspersed homologous sequences (ihss), but only one of five homologous regions (hrs). This suggests that ihss may have an essential role in BmNPV replication.
Key words: nucleopolyhedrovirus; Bombyx mori; serial undiluted
passage; defective genome; BmN4 cell line
Acta virologica 42: 65 – 70, 1998
O. KÚDELA, M. GLASA, E. FUCHS, M. KÚDELOVÁ
Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9,
842 46 Bratislava, Slovak Republic;
Institute for Plant Breeding and Plant Protection – Virology, Martin
Luther University, Halle, Germany
Summary. – Leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (PPV) infection and of peach GF 305 seedlings and Nicotiana benthamiana infected experimentally with PPV were assayed for PPV by polymerase chain reaction (PCR). The expected 243 bp PCR products were subjected to restriction fragment length polymorphism (RFLP) analysis with restriction endonucleases AluI and RsaI. All of the PCR products contained the AluI site. The RsaI restriction profiles of the PCR products demonstrated the prevalence of PPV-M subgroup in the tested samples from western Slovakia.
Key words: plum pox virus; polymerase chain reaction; restriction
fragment length polymorphism; strain variability
Acta virologica 42: 71 – 74, 1998
B. ORZECHOWSKA, M. JANUSZ, B. DOMARACZENKO, Z. BŁACH-OLSZEWSKA
Laboratory of Virology and
Department of Immunochemistry, Institute of Immunology and Experimental
Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53 114 Wrocław,
Poland
Summary. – It is known that resident peritoneal (RP) cells from BALB/c female mice express a constitutive non-specific antiviral immunity which is progressively reduced during several days of cultivation in vitro. In this report, we have studied the effect of a proline-rich polypeptide (PRP) isolated from ovine colostrum on the kinetics of vesicular stomatitis virus (VSV) replication in freshly isolated and one-day cultured RP cells. The polypeptide was added to the cells immediately after virus adsorption or one day before or after viral infection. Independently on time of PRP addition, an inhibition of VSV replication (virus titres reduced by up to 4 log units) was observed. Occasionally, however, a weak stimulation of VSV replication by PRP (virus titres increased by 1–2 log units) was noticed in RP cells constitutively resistant to the infection.
Key words: murine peritoneal cells; proline-rich polypeptide;
ovine colostrum; vesicular stomatitis virus; antiviral effect
Acta virologica 42: 75 – 78, 1998
J. MISTRÍKOVÁ, M. MRMUSOVÁ
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 842 15 Bratislava, Slovak Republic
Summary. – We have followed the effect of murine gammaherpesvirus strain 72 (MHV-72) infection and immunosuppression on the differential white blood cell count of Balb/c mice. In both the acute and chronic phase of infection, abnormal lymphocytes resembling human B lymphocytes infected with Epstein-Barr virus (EBV) were detected. Immunosuppression had no significant effect on the haematological changes during the infection. Some of mice, which had developed tumours as a consequence of infection, showed splenomegaly, lymphadenopathy, leukocytosis and high percentage of immature blastic forms of leukocytes.
Key words: murine gammaherpesvirus; Balb/c mice; differential
white blood cell count; abnormal lymphocytes
Acta virologica 42: 79 – 82, 1998
N. ČEŘOVSKÁ, K. PETRZIK, T. MORAVEC, I. MRÁZ
Institute of Experimental Botany, Academy of Sciences of the Czech
Republic, Prague, Czech Republic;
Institute of Plant Molecular Biology, Academy of Sciences of the Czech
Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic
Summary. – Simple and reliable procedure for sample preparation and reverse transcription-polymerase chain reaction (RT-PCR) detection of potato virus A (PVA) is described. PVA-specific primers used in the RT-PCR defined a target sequence of 321 bp and did not produce amplification product(s) with potato virus Y.
Key words: potato virus A; Nib gene; reverse transcription; polymerase
chain reaction; gel electrophoresis; Southern blot analysis
Acta virologica 42: 83 – 85, 1998
K. PETRZIK, I. MRÁZ, I. DULIĆ-MARKOVIĆ
Department of Plant Virology, Institute of Plant Molecular Biology,
Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České
Budějovice, Czech Republic;
Fruit and Grape Research Centre, Čačak, Federal Republic of Yugoslavia
Summary. – Strawberry vein banding virus (SVBV) was detected by polymerase chain reaction (PCR) and dot-blot hybridisation in samples of cultivated strawberry plants originating from central Slovakia and in samples of wild strawberry plants from south-eastern Serbia in Federal Republic of Yugoslavia (FRY). This is the first finding of SVBV in these countries as well as of SVBV in wild strawberry plants in Europe.
Key words: strawberry vein banding virus; caulimovirus; strawberry;
virus detection; polymerase chain reaction; dot-blot hybridisation
Acta virologica 42: 87 – 89, 1998
M. CHEN, M.Y. FAN, D.Z. BI, J.Z. ZHANG, X.R. CHEN
Department of Rickettsiology, Institute of Epidemiology and Microbiology,
Chinese Academy of Preventive Medicine, 102206 Beijing;
Chinese Academy of Military Medical Sciences, Beijing, People's Republic
of China
Summary. – The nucleotide sequence of rOmpA gene fragment of three Chinese isolates of spotted fever group rickettsiae (SFGR) (BJ-90, Ha-91 and HLJ-054) was determined. The obtained nucleotide and putative amino acid sequences were compared with those of another Chinese SFGR isolate (HL-93) and several prototype SFGR strains. This comparison showed that the isolates BJ-90 and Ha-91 are closely related to each other and R. sibirica but different from the isolates HLJ-054 and HL-93. We assume that with exception of the isolates HLJ-054 and HL-93 that represent new, unique members of SFGR, the isolates BJ-90 and Ha-91 are closely related to R. sibirica, one of the prototype SFGR strains.
Key words: spotted fever group rickettsiae; rOmpA gene; sequence
analysis
Acta virologica 42: 91 – 93, 1998
R.T. WAITE, E.I. SHAW, H.H. WINKLER, D.O. WOOD
Laboratory of Molecular Biology, Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36688, USA
Summary. – The dnaA gene encoding the initiator protein of DNA replication was isolated from the obligate intracellular bacterium, Rickettsia prowazekii. Comparison of the deduced amino acid sequence of R. prowazekii DnaA with other bacterial DnaA proteins revealed extensive similarity. However, the rickettsial sequence is unique in the number of basic lysine residues found within a highly conserved portion of the putative DNA binding region, suggesting that the rickettsial protein may recognize a DNA sequence that differs from the consensus DnaA box sequence identified in other bacteria. Consensus DnaA box sequences, found upstream of many bacterial dnaA genes, were not identified upstream of rickettsial dnaA gene. In addition, gene organization within this region differed from that of other bacteria. The putative start of transcription of the rickettsial dnaA gene was localized to a site 522 nucleotides (nt) upstream of the DnaA start codon.
Key words: Rickettsia prowazekii; dnaA gene; initiator
protein
Acta virologica 42: 95 – 101, 1998
J. RAJČÁNI, A. VOJVODOVÁ
Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 46 Bratislava, Slovak Republic
Summary. – At least nine of the eleven herpes simplex virus (HSV) glycoproteins so far known have been widely characterised as regards their role in the virus replication cycle. During early virus-to-cell adsorption („adsorption"), glycoprotein C (gC) interacts with the glycosoaminoglycan (GAG) heparan sulphate (HS), located on the cell membrane surface. This interaction is labile until other glycoproteins such as B and D (gB and gD) begin to participate in the entry process. gB also harbours a site for interaction with GAGs, while gD provides a stabile attachment to cellular receptors („receptors") such as the herpesvirus entry mediator (HVEM). Late adsorption is associated with a conformation change of gD occurring after the receptor binding, a step followed by interaction of gD with the gH/gL heterodimer (complex). Fusion domains of the gH/gL complex and gB enable the pH-independent virus-into-cell penetration („penetration"). The gE/gI complex and gM interact with the receptors at cell junctions in order to facilitate cell-to-cell spread of the virus along the basolateral surface of polarised cells and/or a similar intercellular spread in nonpolarised cells by avoiding virion release. gK, the only so far known HSV-coded glycoprotein which is not incorporated into virions, plays an essential role in the virus capsid envelopment at the nuclear membrane and in the virion transport to the cell surface. Unusually large polykaryocytes arise due to mutations in syn (syncytium) loci of the viral genome, which were mapped to UL53 (syn1) and UL27 (syn3) genes coding for gK and gB, respectively, while the genes UL20 and UL24 (both syn5) code for nonglycosylated cell membrane-associated proteins („membrane proteins"). The products of nonmutated syn genes either downregulate the fusion of plasma membranes of infected cells („membrane fusion") or protect them from undesirable fusion events.
Key words: herpes simplex virus; glycoproteins; cell receptors;
adsorption; penetration; egress; syncytium
Acta virologica 42: 103 – 118, 1998
Z. HUBÁLEK, J. HALOUZKA, Z. JUŘICOVÁ, O. ŠEBESTA
Institute of Landscape Ecology, Academy of Sciences of the Czech
Republic, Květná 8, 60365 Brno, Czech Republic;
District Public Health Station, 69001 Břeclav, Czech Republic
Acta virologica 42: 119 – 120, 1998
J. DRÁBEK, M. PETŘEK
Department of Immunology, Palacký University and University Hospital, I.P. Pavlova 6, 775 20 Olomouc, Czech Republic
Acta virologica 42: 121 – 122, 1998
V. MAYER, A. MARCELIN, N. DUPIN, M. HURAUX, M. K. BAUM, V. CALVEZ
Institute of Preventive and Clinical Medicine, National Reference
Centre for HIV/AIDS Prevention, Limbová 14, 83301 Bratislava, Slovak Republic;
Department of Virology, Pitié-Salpetriere Hospital, Paris, France;
Department of Dermatology, Tarnier Hospital, Paris;
Department of Psychiatry and Behavioral Sciences, University of Miami,
School of Medicine, Miami, FL, USA
Acta virologica 42: 123 – 124, 1998